From Lonely Planet:
The Vatican After Hours Tour gives visitors the opportunity to view the highlights of its extensive art collection and the Sistine Chapel in small groups of 12 people, three hours after general admission entrance has closed. An expert guide will take the guests on a starlit adventure, exploring a maze of globally-acclaimed art and historic architecture, including works like Raphael’s “School of Athens” and ‘The Raphael Rooms,” and Michelangelo’s “The Creation of Adam” and “The Last Judgement.”
Comprised of 54 galleries, the Vatican Museums offer the crème de la crème of art and architecture in a nocturnal ambience that few ever get to experience. The guides will tell visitors stories of the artists and their lives, along with the rivalry and drama that unfolded between the creators of the Vatican’s most esteemed jewels. They will provide invaluable insights behind the works of art and will delve into some of the most forward-thinking art techniques of the times.